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Supply & Characterization03 / 07
Specialist serviceAntibody Characterization
Fusion Antibodies provides a robust portfolio of antibody characterisation services designed to generate high-quality data that supports confident decision-making across discovery, development, and translation. Our services are available as stand-alone studies or fully integrated with antibody generation, engineering, and reformatting programmes.
30+ years of antibody expertise250+ humanization projectsOne integrated scientific team
Published from Fusion CMS
Antibody Characterization
Fusion Antibodies provides a robust portfolio of antibody characterisation services designed to generate high-quality data that supports confident decision-making across discovery, development, and translation. Our services are available as stand-alone studies or fully integrated with antibody generation, engineering, and reformatting programmes.
In vitro antibody analysis services
Target Binding and Functional Assessment
- Surface Plasmon Resonance (SPR) (including kinetic analysis)
- Bio-Layer Interferometry (BLI) (including kinetic analysis)
- ELISA
- Flow cytometry
Antibody Quantification
- UV/Vis Spectroscopy
- IgG quantification by bio-layer interferometry
- Chromatographic methods
Purity & Contaminant Analysis
- SDS PAGE analysis
- Residual protein A, host cell proteins and DNA assays
- Endotoxin analysis
Aggregation Analysis
- Size exclusion chromatography analysis (SEC)
- Hydrophobic interaction chromatography analysis (HIC)
Charge Variants Analysis
- Ion exchange chromatography analysis (IEC)
Effector Function Characterisation
- FC receptor binding analysis by ELISA
- FC receptor binding analysis by bio-layer interferometry
Stability Assessment
- Freeze-thaw stress testing
- Accelerated degradation studies
- Differential scanning fluorimetry analysis (DSF)
In silico antibody analysis services
Fusion Antibodies offer a suite of in silico antibody analysis services, which uses cutting edge software to examine the primary sequence and tertiary structure of your antibody or recombinant protein. These analyses provide early insight into immunogenicity risk, stability, and manufacturability, helping to de-risk development before costly experimental stages.
Antibody Immunogenicity Screenning
- Evaluate the potential of your antibody to provoke an immune response in patients by screening for T-cell epitopes
- Compare your antibody to all known germlines for similarity.
Primary Antibody Sequence Liability Analysis
Screen for sequence liabilities which can result in complications in downstream manufacturing.
- Isomerization site analysis
- Free cysteine analysis
- Charge variant analysis
- Glycosylation site analysis
- Deamidation site analysis
- Oxidation site analysis
- Proteolytic site analysis
Antibody Stability Analysis
- Evaluation of stability under varying conditions
- Determination of theoretical isoelectric point (pI)
Antibody Aggregation And Hydrophobicity Profiling
Identification of aggregation-prone regions
- Hydrophobicity mapping to support stability optimisation
- Reduced risk of manufacturing challenges and immunogenicity
Move Forward with Confidence
Our characterisation services are designed to deliver actionable insight, not just data, helping you progress antibodies with greater confidence and fewer surprises.
For more information about antibody characterization
A connected workflow
Start with the biology, share the context and move forward with confidence.
Questions, answered
Can this capability connect to another Fusion service? ＋
Yes. Our teams are designed to share context across discovery, engineering and supply.
Tell us what you’re working on.
Share the useful starting details and your enquiry will be sent securely to Fusion’s scientific team.
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